SP600125 (molecular weight = 220) is poorly soluble in water. Stock solutions of at least 20 mM can be made by using 100% dimethyl sulfoxide. As a general guide, for every 10 μM SP600125, it is recommended to include 0.1% DMSO in the culture media (e.g., 30 μM/0.3% DMSO). Prewarming of media and the use of serum protein may enhance solubility. SP600125 typically precipitates as fine needles that are visible at 50-fold magnification.The % of DMSO that is cytotoxic to cells is generally 0.1% and above. This does depend upon the cells you are using. ie macrophages more resistant than epithelial cell lines, primary cells are exquisitely sensitive to levels of DMSO
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