求助各位朋友大神帮我看一下这个
流动相是怎么配置的，梯度是怎么设置的，我不理解，非常感谢！！Separation was achieved using a gradient of mobile phases C and D containing 10 mM ammonium acetate in 10 % ACN (pH= 9.0) and 90 % ACN(pH=9.0), respectively. The flow rate was kept at 0.4 mL/min during a 12-min run with the following gradient: 99 % D for 0.5 min to 60 % D at 6.0 min to 20 % D at 8 min. The gradient was held at 20 % D for 1 min then returned to 99 % D for 2 min for column equilibration before subsequent injections.
